"We will not enter through these metal detectors", al-Qiswani said, according to AFP.
Police said some worshippers refused to go through them and knelt to pray outside instead.
Israeli police said the armed attackers had been spotted around 7 a.m. local time approaching the gateway from within the sacred compound which Jews revere as the Temple Mount and Muslims as the Noble Sanctuary, The New York Times reported.
The American ambassador to Israel, David Friedman, wrote on Twitter about the attack: "Shocked and horrified by the despicable attack".
"This is a severe violation of the status quo", said Shikh Omar al-Qiswani, the director of the al-Aksa Mosque, said in a statement.
"We will not break the solidarity of the people", said Jamal Abdallah, a Palestinian who now lives in the USA state of Arizona and was planning to visit Al-Aqsa, but changed his mind when he was told of the situation.
Palestinian President Mahmoud Abbas condemned the attack in a phone call with Israeli Prime Minister Benjamin Netanyahu, but also said closing down the area could have repercussions.
A rash of Palestinian attacks on Israeli civilians and soldiers erupted in 2015, partly over tensions at the holy site.
The two dead Israeli Border Police officers were members of Israel's small Druze community.
Israeli authorities also detainedJerusalem's top Islamic cleric as crowds gathered at the Old City's gates after the attack, his son said.
Gaza's terrorist Hamas rulers on Saturday called on Palestinians to attack Israeli forces in Yerushalayim after the Har Habayis was closed following a terrorist attack there.
There was no immediate comment from Palestinian officials.
Israel says at least 175 of those killed were carrying out attacks while others died in clashes and protests. It is the holiest site in Judaism and the nearby Western Wall, a remnant of one of the temples, is the holiest place where Jews can pray. Israel has assured the world that it has no intention to alter the status of this holy site, a decision which the United States applauds and welcomes.
The spokesperson warned of the impact of this incident on the peace process, as well as reviving efforts and negotiations between Palestinians and Israelis. The violenceclaimed several thousand victims, a lot of them Palestinians, and only began to ebb in 2005.
Impegnati nelle opere di spegnimento un canadair, tre elicotteri e sei squadre dei vigili del fuoco con autobotti. Le fiamme stanno lambendo alcune abitazioni che per questo motivo sono state evacuate.
Il lotto ritirato era destinato sia al mercato della grande distribuzione organizzata, sia alle società di servizi di catering . Scatta l'allarme per i consumatori del tonno all'olio di girasoli dopo il richiamo da parte del Ministero della Salute .
Veniva presentata una rendicontazione artefatta per poter lucrare su delle spese che in realtà erano di gran lunga inferiori. Addirittura uno degli indagati con questi fondi ha portato tutta la famiglia in Spagna a villeggiare ".